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. Author manuscript; available in PMC: 2016 Dec 1.
Published in final edited form as: Mol Cancer Res. 2015 Aug 13;13(12):1591–1601. doi: 10.1158/1541-7786.MCR-14-0626

Figure 3. ABC294640 negatively regulates CRPC signaling and proliferation.

Figure 3

A, C4-2 cells were cultured in hormone-proficient media and treated with 10 μM, 20 μM ABC294640 or vehicle for 24 and 48 hours. Cell lysates were immunoblotted with AR, Myc, and GAPDH. B, C4-2 cells were cultured in hormone-depleted media and treated with 20 μM ABC294640 or vehicle for 72 hours and mRNA transcripts levels of Myc, CDK1, and ODC were analyzed by qRT-PCR. C, C42 cells were cultured as in B and mRNA transcripts levels of AR, PSA, TMPRSS2, FKBP5 were analyzed by qRT-PCR. D, C4-2 cells were cultured in hormone-proficient media and treated with 10 μM (●), 20 μM (▲) ABC294640 or vehicle (■) for 72 hours. Cell growth was determined by trypan blue exclusion cell counting. *p < 0.05, **p < 0.01.