Skip to main content
. Author manuscript; available in PMC: 2017 Jan 1.
Published in final edited form as: J Immunol. 2015 Nov 20;196(1):310–316. doi: 10.4049/jimmunol.1501836

FIGURE 9. Irf7 is required for generation of anti-pH1HA adaptive immune responses.

FIGURE 9

WT and Irf7−/− mice were vaccinated intramuscularly with pH1HA. (A) HA-specific IgG titers, including their isotypes (B), were analyzed 14 days post second vaccination. Anti-HA binding avidity was quantified via ELISA and reported as molar concentration of sodium thiocyanate required to displace anti-HA serum antibodies to 2x pre-bleed levels (C). Splenocytes and bone marrow were harvested at termination 7 days following the third boosting vaccination. (D) Splenocyte B cells were plated immediately following isolation, while (E) bone marrow cells were plated after 5 days of culturing in non-specific stimulation to promote clonal expansion. (F) Frequency of HA peptide-specific IFN-γ+ T cells in mice immunized with pH1HA. Data are the averages ± SEM of 2 independent studies of 5 mice per group (Total N=10 per phenotype). **p<0.01, ***p<0.001 versus control group.