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. 2015 Dec 15;408(2):213–228. doi: 10.1016/j.ydbio.2015.03.008

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© 2015 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 5 — Myeloid cells migrate preferentially towards the wound site after injury. (A) Schematic representation of a biopsy punch wound assay in lurp1:GFP transgenic Xenopus larva in vivo. The perimeter (red circle) and the area (gray circle) of the excisional wound are indicated. (B) Averages of the area (gray bar) and the perimeter (red line bar) of the wounds measured from 3 independent experiments. (C) One channel confocal projection of the starting point of migration of the myeloid GFP+ cells (gray scale) merged with the cell tracks obtained from the time-lapse analysis. The wound site is highlighted in yellow. See supplementary video 10, associated with this panel. (D) The cell tracks were plotted forcing a common origin in a quadrant mesh. (E) and (F) The magnitude and the angles of the displacement vectors obtained from the GFP+ cell tracks after biopsy punch wounding assay were plotted in rose charts (MatLab). The location of the wound site is denoted by a pink overshadow. Rayleigh test (CircStat for MatLab, The MatWorks) p-value is also indicated. (G) The speed of migration was calculated for each GFP+ cell following light stimulation (unwounded, green dots) or after a biopsy punch-wounding assay (wounded, red dots). The mean speed is indicated with a transversal black line. Mann–Whitney U-test, ***p<0.001. (H) and (I) The magnitude and the angles of the displacement vectors obtained from the GFP+ cell tracks after light stimulation were plotted in rose charts (MatLab). Rayleigh test (CircStat for MatLab, The MatWorks) p-value is also indicated.