Fig. 8.

Macrophage response to bacterially infected wounds. (A) Schematic representation of the wounding assay plus bacteria infection in mpeg1:GFP transgenic Xenopus larva. The puncher was loaded with live E. coli (red tip) before generating the excisional wounds. (B) Single-channel confocal projection merged with the best in focus transmitted light image of the infected wound. The excised tissue area is highlighted in red. GFP+ cells (Macrophage-like) that stopped at the infected wound margin (blue arrowheads) and at the periphery (red arrowheads) are also indicated. See supplementary video 13, associated with this panel. (C) Average speed was calculated from the tracks of mpeg+ cell from at least 3 different experiments for each condition (3 h after wounding). Mann–Whitney U-test, * p<0.05. (D) Point speeds of migration (solid lines) were traced for the cells pointed with blue arrowheads in the panel B. (E) Point speeds of migration (solid lines) were traced for the cells denoted with red arrowheads in the panel B.