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Transformation efficiency of B. subtilis 168 (wt) and REG6 (PmtlA-comK). Cells were transformed according to Paris method using Spizizen's minimal medium containing glucose (MG) and the new protocol using LB medium with(out) mannitol. 100 ng of the pWAL275 dimers (A) and 3 μg of KM0 chromosomal DNA (B) were used for transformation. Error bars represent standard deviation from the mean value of triple experiments.
