Table 1.
Strain | Genotype | Source, Reference, or Construction |
---|---|---|
E. coli | ||
JM109 | recA1, endA1, gyrA96, thi-1, hsdR17(, ), mcrA, supE44, gyrA96, relA1, λ−, Δ(lac-proAB), F′ (traD36, proAB+, lacIq, lacZΔM15) | Yanisch-Perron et al., 1985 |
NM538 | supF, hsdR, trpR, lacY | Frischauf et al., 1983 |
B. subtilis | ||
168 | trpC2 | DSM23778* |
IIG-Bs168-1 | trpC2, ΔmanPA::ermC | pJOE6577.1 → 168 |
KM0 | trpC | pKAM041 → 168 |
REG1 | trpC2, ΔmanPA::ermC, hisI′-spcR-yvcA | pHM30 → IIG-Bs168-1 |
REG3 | trpC2, ΔmanPA::ermC, PmtlA-comS | pREG3 → REG1 |
REG4 | trpC2, ΔmanPA::ermC, PmtlA-dprA | pREG4 → REG1 |
REG5 | trpC2, ΔmanPA::ermC, PmtlA-dprA-comS | pREG5 → REG1 |
REG6 | trpC2, ΔmanPA::ermC, PmtlA-comK | pREG6 → REG1 |
REG7 | trpC2, ΔmanPA::ermC, PmtlA-comK-dprA | Gibson 1 → REG1 |
REG19 | trpC2, ΔmanPA::ermC, PmtlA-comK-comS | pJOE7361.1 → REG1 |
REG32 | trpC2, ΔmanPA::ermC, PmtlA-comK-comS, ΔcomS | pREG12 → REG19 |
REG33 | trpC2, ΔmanPA::ermC, PmtlA-comK-comS, ΔcomK | pREG13 → REG19 |
REG35 | trpC2, ΔmanPA::ermC, PmtlA-comK-comS, ΔcomK, ΔcomS | pREG13 → REG32 |
REG36 | trpC2, ΔmanPA::ermC, PmtlA-comK-comS, Δspo0A | pJOE7122 → REG19 |
REG37 | trpC2, ΔmanPA::ermC, PmtlA-comK-comS, ΔcomQXPA | pREG10 → REG19 |
REG120 | trpC2, ΔmanPA::ermC, PmtlA-comK-comS, ΔdegU | pREG58 → REG19 |
Deutsche Sammlung von Mikroorganismen und Zellkulturen.