Table 1.

List of primers for detection of Klebsiella pneumoniae virulence genes.

Gene	Primer	Primer sequence	Amplicon (bp)	Annealing temperature (°C)	References
K1	K1-F	GTAGGTATTGCAAGCCATGC	1047	55	Fang et al., 2007
	K1-R	GCCCAGGTTAATGAATCCGT
K2	K2-F	GACCCGATATTCATACTTGACAGAG	641	57	Turton et al., 2008
	K2-R	CCTGAAGTAAAATCGTAAATAGATGGC
K5	K5-F	TGGTAGTGATGCTCGCGA	280	55	Turton et al., 2008
	K5-R	CCTGAACCCACCCCAATC
K20	K20-F	CGGTGCTACAGTGCATCATT	741	55	Fang et al., 2007
	K20-R	GTTATACGATGCTCAGTCGC
K54	K54-F	CATTAGCTCAGTGGTTGGCT	881	55	Fang et al., 2007
	K54-R	GCTTGACAAACACCATAGCAG
K57	K57-F	CTCAGGGCTAGAAGTGTCAT	1037	55	Fang et al., 2007
	K57-R	CACTAACCCAGAAAGTCGAG
wcaG	wcaG-F	GGTTGGKTCAGCAATCGTA	169	53	Turton et al., 2010
	wcaG-R	ACTATTCCGCCAACTTTTGC
rmpA	rmpA-F	ACTGGGCTACCTCTGCTTCA	535	50	Nadasy et al., 2007
	rmpA-R	CTTGCATGAGCCATCTTTCA
magA	magA-F	GGTGCTCTTTACATCATTGC	1282	53	Fang et al., 2004
	magA-R	GCAATGGCCATTTGCGTTAG
Aerobactin	Aerobactin-F	GCATAGGCGGATACGAACAT	556	55	Yu et al., 2008
	Aerobactin-R	CACAGGGCAATTGCTTACCT