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. 2015 Dec 21;6:1136. doi: 10.3389/fpls.2015.01136

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Copyright © 2015 He, Liu, Zheng, Cui, Shen and Zheng.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Validation of RNA-Seq data by real-time qRT-PCR or RT-PCR. (A) Validation of several differentially expressed Cd up- or down-regulated genes by qRT-PCR. (B) qRT-PCR detection of long non-coding RNAs expression under Cd stress. (C) Semi-quantitative PCR detection of alternative splicing event analysis under Cd stress. Rice seedling roots were collected 24 h after 10 μM Cd (Cd+) exposure in (A,C), or treated with 0.1, 1, 10, and 100 μM Cd for (B), see details in the Materials and Methods.