Figure 1.

Incremental reduction in the complexity of a process for plant extract clarification. (A) The clarification process implemented for the manufacture of the plant-derived antibody 2G12 under GMP conditions in 2009 consisted of a total of five filtration steps, including three depth filters. (B) The filter train complexity was reduced as part of a new production campaign in 2013/2014, now consisting of three filtration steps and two multi-use components. (C) In the current process, a pre-coat filtration step replaces the bag and depth filters, reducing both the number of filtration steps and the number of multi-use components.