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. 2015 Aug 21;61(6):519–524. doi: 10.1262/jrd.2015-073

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Fig. 1. — Relationship between acrosomal integrity and distributive normality of acrosomal tyrosine-phosphorylated (pY) proteins in frozen-thawed spermatozoa from Japanese Black bulls. Filled circles (●) indicate the results obtained for each of 34 bulls (A1–A9, B1–B9, C1–C9 and D1–D7). ^a Acrosomal integrity was examined by the fluorescein isothiocyanate (FITC)-conjugated peanut agglutinin (PNA)/propidium iodide (PI) staining. ^bDistributive normality of acrosomal pY proteins was examined by indirect immunofluorescence.