Fig. 2.

Survival of immature cumulus-enclosed porcine oocytes after vitrification in different combinations of penetrating CPA. Four replications were performed. Total numbers of oocytes subjected to each treatment are given in parentheses (n). Data are presented as mean ± SEM values. ^{a, b, c} Percentages with different letters in the same column differ significantly (P < 0.05). After warming, all vitrified COCs were subjected to IVM and IVF. Viability status of oocytes was evaluated after IVF. Live oocytes were subsequently used either for assessment of nuclear and fertilization status or for embryo culture (results are given in Tables 4 and 5, respectively).