. 2015 Sep 27;61(6):571–579. doi: 10.1262/jrd.2015-089

Table 6. Survival after warming and IVM and subsequent in vitro embryo development after IVF of immature cumulus-enclosed oocytes vitrified by different equilibration regimens.

Treatment	CPA treatment regimen	CPA	Total	Live* (% total)	Cleaved embryos (% live)	Blastocysts (day 6)
Treatment	CPA treatment regimen	CPA	Total	Live* (% total)	Cleaved embryos (% live)	(% live)	(% cleaved)	(% total)
Control	-	-	310	307	194	62
Control	-	-	310	(99.0 ± 0.5)^a	(63.4 ± 4.6)^a	(20.3 ± 2.0)^a	(32.0 ± 2.2)^a	(20.1 ± 1.9)^a
Vitrified	Regimen A	EG + PG	368	304	73	10
Vitrified	Regimen A	EG + PG	368	(82.5 ± 4.6)^b	(24.0 ± 4.5)^b	(3.2 ± 0.1)^b	(13.6 ± 0.9)^b	(2.7 ± 0.2)^b
Vitrified	Regimen B	EG + PG	363	80	7	0
Vitrified	Regimen B	EG + PG	363	(22.4 ± 4.3)^c	(7.6 ± 3.8)^c	(0 ± 0)^c	(0 ± 0)^c	(0 ± 0)^c
Vitrified	Regimen B	EG + DMSO	350	92	29	3
Vitrified	Regimen B	EG + DMSO	350	(26.6 ± 4.5)^c	(31.5 ± 1.6)^b	(2.8 ± 1.6)^b	(9.4 ± 5.8)^b	(0.9 ± 0.5)^c

Three replications were performed. Data are presented as mean ± SEM values. * After IVF, all live oocytes were subsequently cultured for 6 days (day 0 = IVF). ^{a, b, c} Percentages with different letters in the same column differ significantly (P < 0.05). Regimen A = equilibration in a total of 4% (v/v) CPA for 15 min followed by a brief (approx. 40 sec) washing and vitrification in 35% (v/v) CPA. Regimen B = equilibration in a total of 15% (v/v) CPA for 5 min followed by a brief (approx. 40 sec) washing and vitrification in 35% (v/v) CPA. EG + PG = ethylene glycol + propylene glycol (1:1). EG + DMSO = ethylene glycol + dimethyl sulfoxide (1:1).