Table 7. Survival after warming and IVM and subsequent in vitro embryo development after IVF of immature cumulus-enclosed oocytes vitrified in a total of 35% (v/v) CPA (EG + PG = 1:1) after different durations of equilibration in a total of 4% (v/v) CPA.

Treatment	Equilibration duration	Total	Live (% total)	Cleaved embryos (% live*)	Blastocysts (day 6)
					(% cultured)	(% cleaved)	(% total)
Vitrified	5 min	418	260	65	14
			(62.5 ± 4.6)	(25.3 ± 4.6)	(5.6 ± 2.0)	(33.1 ± 4.7)	(3.4 ± 1.2)
Vitrified	15 min	432	302	68	10
			(70.1 ± 3.2)	(22.4 ± 3.9)	(3.3 ± 0.9)	(19.1 ± 2.8)	(2.3 ± 0.6)
Vitrified	25 min	440	315	69	7
			(71.8 ± 3.1)	(21.9 ± 1.0)	(2.3 ± 1.0)	(13.2 ± 5.9)$	(1.6 ± 0.6)

Four replications were performed. Data are presented as mean ± SEM values. * After IVF, all live oocytes were subsequently cultured for 6 days (day 0 = IVF). ^$ Tendentious difference compared with vitrified oocytes equilibrated for 5 min (P = 0.08).