Figure 4. T cells are recruited to the airways and lungs of MTM^−/− mice after RSV infection.

MTM^−/− and wt mice were intranasally administrated PBS or infected with 2 × 10⁶ FFU of RSV and at day 8 post infection BAL and lungs were analysed. (A) Total number of BAL cells was quantified and the frequency and quantity of lymphocytes were determined using differential cell counting of H&E stained cytospin slides. (B) Frequencies and absolute numbers of CD8⁺ and CD4⁺ T cells in the BAL were determined by flow cytometry on day 8 after PBS administration or RSV infection. (C) Total cells and frequencies and numbers of CD8⁺ and CD4⁺ T cells were quantified in the lungs of wt and MTM^−/− mice at day 8 p.i. using flow cytometry. Data are shown as mean ± SEM of 7–9 mice per group, pooled from two individual experiments. ***p ≤ 0.001, **p ≤ 0.01.