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. 2015 Dec 21;5:18519. doi: 10.1038/srep18519

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Copyright © 2015, Macmillan Publishers Limited

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(A) MKs were enriched by density-gradient centrifugation. The 50% layer was collected and stained with MGG. (B) Enriched MKs were cultured in the presence or absence of xlTPO. After two days suspension culture in the absence of xlTPO, spindle-shaped thrombocyte-like cells were observed (solid arrowheads and inset). Bars represent 20 μm. (C) The morphology of cultured hepatic cells. Enriched MKs were cultured for two days in the presence or absence of xlTPO; hepatic cells were cytocentrifuged onto slide glass and stained with T12. (D) The morphology of peripheral thrombocytes in suspension after two days (inset). (E) Whole cultured thrombocytes were incubated with or without thrombin, and the proportion of spindle-shaped thrombocytes was calculated. Left panels show the changing morphology of cultured thrombocyte-like cells. Graphs represent means + SD, n = 6. *P < 0.05 vs. thrombin^-.