Figure 2. PCGF1 co-purifies with members of the variant PCGF1-PRC1 complex, as well as additional proteins linked to diverse cellular processes.

(A) Proteins enriched by PCGF1 immunoprecipitation are shown in Volcano diagrams that plot t-test difference versus P-value for enrichment in IgG or PCGF1 immunoprecipitation experiments. Bait (PCGF1) is shown in red, previously described members of vPRC1 in green, and pluripotency factors in blue. (B) Mass spectrometry protein coverage and previous reports in the literature for PCGF1-interacting proteins. (C) Co-immunoprecipitation experiments whereby PCGF1, BCOR and DPPA4 are pulled-down and probed with antibodies to PCGF1, BCOR, DPPA4 and BMI1. D. Network visualization of the PCGF1 interactome. Functional categories were defined using the Cytoscape plugin BiNGO²².