Figure 4. A pluripotency-associated sub-network linked to PCGF1.

(A) Volcano diagrams for immunoprecipitations of BCOR, DPPA4, NANOG and OCT4. Baits are shown in red, previously described members of vPRC1 in green, and pluripotency factors in blue. (B) Network diagram of reciprocal and non-reciprocal physical interactions detected in affinity purification mass spectrometry experiments with the tail of the arrow on the immunoprecipitated protein. (C) Co-immunoprecipitation of PCGF1, DPPA4, NANOG, OCT4, BCOR, and RNF2 with western blot staining for PCGF1, PCGF2, BMI1, BCOR, RNF2, DPPA4, NANOG and OCT4.