Table 2. Experimental runs for RSM, created by utilizing CCD. In pattern, A and a means the highest and lowest axial levels, respectively.

Run	pattern	Col I (mg/mL)	LN (mg/mL)	FN (mg/mL)
1	−−−	0.21	0.21	0.09
2	−−+	0.21	0.21	0.33
3	−+−	0.21	0.83	0.09
4	−++	0.21	0.83	0.33
5	+−−	0.83	0.21	0.09
6	+−+	0.83	0.21	0.33
7	++−	0.83	0.83	0.09
8	+++	0.83	0.83	0.33
9	a00	0.00	0.52	0.21
10	A00	1.04	0.52	0.21
11	0a0	0.52	0.00	0.21
12	0A0	0.52	1.04	0.21
13	00a	0.52	0.52	0.00
14	00A	0.52	0.52	0.42
15	000	0.52	0.52	0.21