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. 2015 Dec 21;5:18709. doi: 10.1038/srep18709

Figure 3. Histone demethylase KDM7A is important for G9a-mediated silencing of E-cadherin.

Figure 3

(A) Expressions of three H3K27 demethylases KDM6A, KDM6B and KDM7A in control or G9a-depleted PANC-1-R cells were determined by RT-qPCR analysis. *p < 0.05. (B) The protein levels of G9a, E-cadherin and KDM7A in PANC-1-R cells transfected with control or various G9a shRNAs was investigated by Western blot analysis. Actin was served as a loading control. (C) The protein levels of KDM7A, E-cadherin, G9a and EZH2 in PANC-1-R cells transfected with control or KDM7A expression vectors were investigated by Western blot analysis. (D) PANC-1-R cells received different combined transfection of G9a or KDM7A shRNAs were harvested for Western blot analysis to determine the protein levels of G9a, E-cadherin and KDM7A. (E) PANC-1-R cells were treated with different doses of UNC0638 for 48 h and KDM7A protein level was studied. (F) The binding of G9a and MLL to human KDM7A promoter and the methylation status of KDM7A promoter were investigated by ChIP-qPCR. Results are averaged data of two independent experiments.