Table 1.

Antibodies used for immunohistochemistry and immunocytochemistry

Protein	Function	Catalog No.	Dilution
Thrombotic proteins
von Willebrand factor	Binds platelets to form platelet thrombi when in ultralarge multimer form	Ab6994*	1:200
Tissue Factor	Surface protein expressed by activated endothelial cells to initiate coagulation cascade	SC18712†	1:50
Plasminogen activator inhibitor 1	Tissue plasminogen activator inhibitor	SC6642†	1:100
Antithrombotic proteins
A disintegrin and metalloproteinase with a thrombospondin type I motif, member 13	von Willebrand factor cleaving enzyme	BL-159‡	1:500
Tissue factor pathway inhibitor	Major inhibitor of tissue factor, factor Xa, and thrombin	SC18712†	1:100
Tissue plasminogen activator	Regulator of fibrinolysis	Bs-1545R§	1:50
ECM proteins
Collagen type I	Fibrillar collagen concentrated at fibrosa of AV leaflet; most abundant AV ECM component	Ab34710*	1:50
Collagen type II	Fibrillar collagen found mainly in fibrosa of AV leaflet	Ab7778*	1:500
Elastin	Highly elastic ECM component concentrated at ventricularis of AV leaflet	Ab9519*	1:50

AV indicates aortic valve; and ECM, extracellular matrix

Corresponding manufacturers:

^*Abcam (Cambridge, MA)

^†Santa Cruz Biotechnology (Santa Cruz, CA)

^‡Bethyl Laboratories (Montgomery, TX)

^§Bioss Laboratories (Woburn, MA)