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. 2015 Dec 1;34(6):390–395. doi: 10.1089/mab.2015.0042

FIG. 2.

FIG. 2.

Investigation of sortilin expression in a number of cell lines using commercial rabbit anti-sortilin antibody in Western blot analysis. All cells expressed the main isoform (NP_002950.3, 833 aa, ∼100 kDa) of sortilin protein except the mouse embryonic fibroblast cell line, NIH/3T3, which was negative for sortilin expression in the Western blot. Presence of some low-molecular-weight bands in HEK-293 and PC3 cell lines may represent the expression of smaller sortilin protein isoforms such as NP_001192157 (694 aa, ∼80 kDa).