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. 2015 Dec 1;34(6):390–395. doi: 10.1089/mab.2015.0042

FIG. 3.

FIG. 3.

(A) Detection of sortilin expression in NIH/3T3-sortilin (stably sortilin-transfected NIH/3T3) using reactivity with commercial rabbit anti-sortilin antibody in Western blot analysis. Commercial antibody recognized 100 kDa band of sortilin in lysates of Caov-4 (positive control) and NIH/3T3-sortilin but not in NIH/3T3-vector (stably pCMV6-Neo-transfected NIH/3T3). (B) Reactivity of 2D8 to human sortilin molecule by Western blot analysis. 2D8 recognized human sortilin as a 100 kDa band in the lysates of NIH/3T3-sortilin, ovarian carcinoma tissues, and cell lines but not in NIH/3T3-vector. NIH/3T3-sortilin and NIH/3T3-vector provided positive and negative controls, respectively. β-actin protein was detected as internal control in all samples. OC, ovarian carcinoma.