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Indirect immunofluorescence of 2C2D3 and 4H7F4. A431 cells were incubated with rat anti-EGFR antibody (2C2D3 or 4H7F4) prior to fixation. Bound antibodies were detected by Alexa 488-conjugated anti-rat IgG antibody (green). The nuclei were counterstained with Hoechst dye (blue).
