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. 2015 Dec 1;28(6):411–422. doi: 10.1089/jamp.2015.1241

FIG. 2.

FIG. 2.

CryoTEM micrographs of 12.5 mg/mL CFI, 90 mg/mL sucrose, 0.1% polysorbate 20, before and after liquid nitrogen freeze-thaw, and after nebulization. The scale bar in the bottom left-hand corner is 100 nm for all images. All samples were applied at a concentration of ∼10 mg/mL liposomes. (A) Unfrozen control at pH 5.9; (B) pH 5.9 after thawing; (C) pH 5.3 after thawing; (D) pH 5.3 after thawing and nebulization; (E) pH 4.9 after thawing; (F) pH 4.9 after thawing and nebulization. The dashed arrows in D show ice artifacts introduced during sample preparation.