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. 2015 Dec 21;5:285. doi: 10.3389/fonc.2015.00285

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Copyright © 2015 de Groot, Hsia, Anzola, Motamedi, Yoon, Wong, Jenkins, Lee, Martinez, Davis, Gahman, Desai and Shiau.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Measurement of G2 duration provides a specific readout for Aurora A kinase activity. (A) Images from a timelapse sequence of HeLa cells stably expressing GFP-PCNA; H2B-RFP. Gray scale images for the two separate channels are located below each colored merge. G2 duration is measured as the time from dissolution of PCNA foci to nuclear envelope breakdown (NEBD). See also Movie S1 in Supplementary Material.(B) Analysis of G2 duration in HeLa cells following knockdown of Aurora A or Aurora B by RNAi. Blue lines indicate the mean; black error bars are the SD. p-values are from unpaired t-tests. (C) Analysis of G2 duration in HeLa cells using selective Aurora A versus Aurora B inhibition, with MK-5108 (6 μM) and AZD1152-HQPA (100 nM), respectively. Blue lines indicate the mean; black error bars are the SD. p-values are from unpaired t-tests.