Figure 2.

Well-differentiated primary nasal epithelial cultures can be transduced with adenovirus expressing GFP. (a) GFP and (b) bright-field view of well-differentiated primary human nasal epithelial cells transduced at time of seeding onto semipermeable inserts with HD-Ad-GFP. Image was taken after 24 days of air-liquid interface culture. Panel (c) to (h) demonstrates GFP signal in WD-PNECs transduced with HD-Ad-GFP after 21 days in ALI culture. GFP expression was examined day 3 post-transduction. (c) Hundred viral particles per cell were used without any pretreatment. (d to f) Cells were pretreated with apical 6 mmol/l ethylene glycol tetraacetic acid (EGTA) prior to addition of (d) 100, (e) 1,000, and (f) 2,500 viral particles HD-Ad-GFP per cell. (g) and (h) were parallel experiments. Cells were pretreated with (g) 0.01% lysophosphatidylcholine or (h) 6 mmol/l EGTA. ALI, air liquid interface; HD-Ad-GFP, helper-dependent adenovirus expressing green fluorescent protein.