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. 2015 Sep 9;2:15034. doi: 10.1038/mtm.2015.34

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Copyright © 2015 Official journal of the American Society of Gene & Cell Therapy

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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Apical expression of CFTR in cystic fibrosis (CF) cells postadenoviral transduction. (a) Human nasal epithelial cells from p.F508del/p.F508del CF patient in the absence of transduction and (b) post-transduction using HD-Ad-CFTR vector (2,500 viral particles per cell). Vector was added apically to cells after 21 days of air liquid interface culture and pretreatment with ethylene glycol tetraacetic acid. CFTR staining (green) is seen on the apical membrane of cells (white arrows). CFTR, CF transmembrane conductance regulator; DAPI, 4’, 6-diamidino-2-phenylindole.