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. 2015 Sep 9;2:15034. doi: 10.1038/mtm.2015.34

Figure 5.

Figure 5

Functional expression of CFTR in human nasal epithelial cells (HNECs) following treatment HD-Ad-CFTR. Functional CFTR expression in HNEC measured in Ussing chamber studies. Original HNEC Ussing tracings from a CF patient (G480S/A559T) 72 hours after treatment. (a) HD-Ad-GFP or (b) HD-Ad-GFP. For panels (c) and (d), circles represent nontransduced cells, squares represent HD-Ad-CFTR transduced cells, and triangles represent HD-Ad-GFP-transduced cells. (c) Summary of CFTR function measured as Forskolin-activated current (Ieq-FSK) in HNEC from different CF patients, showed no change in CFTR function following treatment with HD-Ad-GFP (n = 4), but significant Ieq-FsK response following HD-Ad-CFTR (n = 7, P = 0.0017). (d) Similarly, the summary of CFTR function expressed as CFTRInh172 sensitive currents (Ieq CFTRInh172) showed no change with HD-Ad-GFP (n = 4), but significant increase in Ieq CFTRInh172 following HD-Ad-CFTR (n = 7, P < 0.001). CF, cystic fibrosis; CFTR, CF transmembrane conductance regulator; HD-Ad-GFP, helper-dependent adenovirus expressing green fluorescent protein.