Table 1.

Effects of CHMI on activities of individual subjects in the DNA/Ad and AdCA trials

	ELISpot	CD8+ T cell IFN-γ	CD8+ T cell IFN-γ EM	Antibody
Vaccine	Status	Vol.	CSP	AMA1	CSP	AMA1	CSP	AMA1	CSP	AMA1
DNA-Ad	P	v06	0	+1.2	0	0	0	0	−1.8	−1.3
		v10	0	−2.01	0	−4.2	0	−11.51	−2.3	−1.4
		v11	−3.21	−2.61	−9.91	−4.51	−28.91	−5.21	−4.0	−4.4
		v18	−1.61	−1.81	−2.41	−2.51	−2.41	−3.01	−1.4	−2.1
	Non-P	v01	+4.9*	+1.8	0	+1.4	0	0	−1.7	+1.1
		v02	0	+3.0	0	0	0	0	−1.2	+2.3
		v03	+1.6	+2.5	−3.5	−2.5	0	0	−2.2	+1.6
		v05	0	+3.3	1.0*	+1.1	0	0	+2.7	+2.5
		v09	0	+4.8*	+2.2*	1.0	0	0	+1.4	+2.0
		v12	+1.8	+1.2	+1.1*	1.0	0	0	1.0	−2.8
		v13	0	+1.4	0	0	0	0	1.0	+4.2
		v15	0	+2.8	−4.4	−1.2	0	0	1.0	+1.1
		v16	0	+4.7	0	−3.3	0	0	1.0	+3.0
		v17	+1.1*	+2.0	+1.45*	−4.0	0	0	+6.7	+3.7
		v19	0	+5.7*	−2.2	−4.2	0	0	−3.6	1.0
Ad-CA	Non-P	v118	−2.1	−1.4	NT	NT	NT	NT	+4.6	+133.3
		v119	−2.1	−3.8	−1.1	−1.7	−1.2	−1.7	+3.9	+2.0
		v125	−1.7	−1.6	1.0	−2.1	0	−1.9	−1.5	+7.1
		v126	−2.4	−1.4	−8.5	−2.2	0	−1.5	+8.5	+74.5
		v127	−1.9	−2.4	−1.2	−3.2	−1.2	−3.2	−1.7	+7.3
		v128	−4.1	−1.9	+1.4	+1.3	NT	+1.4	−1.7	+10.0
		v147	−1.3	−1.2	−2.7	+1.3	−2.1	0	1.0	+146.0
		v149	−1.1	−1.2	NT	NT	NT	NT	+5.8	+9.7
		v156	−2.5	−2.1	−1.2	−2.4	−1.6	−1.0	+1.5	+9.2
		v160	−1.5	−1.3	−3.6	−3.2	−6.5	−23.8	+1.8	+2.1
		v172	−2.0	−1.6	−1.1	−2.7	0	−24.9	+1.1	+7.7
		v173	1.0	−1.3	0	−1.2	0	0	0	+15.3
		v179	−2.4	−1.1	−2.1	−1.9	0	−1.4	−1.4	+28.2
		v184	−4.9	−3.1	−1.7	−1.1	0	0	0	+475.0
		v194	−3.5	−1.9	0	−1.2	0	0	−2.4	+5.1
		v195	0	−4.2	1.0*	−1.5	0	−1.2*	−1.4	+10.1
		v196	−2.9	−1.8	−1.2	−2.6	0	−2.4	+2.0	+16.2

The effects of CHMI on pre-CHMI cellular and antibody activities are shown for each volunteer in the DNA/Ad and AdCA trials. The fold-change of pre- and post-CHMI activities is shown as a rise (+) or fall (−). The threshold for significance was fold-changes >±1.5, which was the range of changes in non-immunized infectivity controls: rises are shown in blue boxes, and falls are shown in green boxes.

¹Fold-changes of responses to immunodominant peptide pools.

^*Activity was negative pre-CHMI but was positive post-CHMI. 0: Pre-CHMI and post-CHMI activities were negative.