Glial Fibrillary Acidic Protein (GFAP) immunofluorescence, neuronal nuclei (NeuN), and HE staining in the contralateral (A, B, C) and in the FUS-BBB opened brain (D, E, F). Neuron distributions (nuclei stained by NeuN; B and E) appeared similar on both sides of the brain, but concentrations of activated glial cells (stained by GFAP; A and D) were increased in the experimental lateral brain. HE-staining (C and F) showed that the tissue structure was not severely damaged by FUS treatment. Bar = 200 μm.