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. Author manuscript; available in PMC: 2015 Dec 21.
Published in final edited form as: Compr Physiol. 2011 Apr;1(2):971–993. doi: 10.1002/cphy.c100053

Figure 10.

Figure 10

Effect of prolylcarboxypeptidase (PRCP) blockade by a siRNA on bradykinin (BK) release in Ad-AT2R-transfected mouse coronary endothelial cells (ECs). (A) Representative Western blot showing PRCP protein expression (top) and quantitative analysis of PRCP protein (bottom); PRCP SiRNA, but not scarmbled-SiRNA (S-siRNA) significantly blocked PRCP expression. (B) Fold change in bradykinin release relative to Ad-GFP-transfected cells; BK release was further increased when Ad-AT2R-transfected ECs were treated with CGP42112A, a selective AT2 agonist (CGP; 0.1 μmol/l). SiRNA, but not S-siRNA significantly blunted Ad-AT2R-induced bradykinin release, n = 4. [Reprinted from Zhu, Carretero, Yang et al., (318).]