Table 1. Real Time PCR Primer Sequences.
| Gene | Tm (°C)a | Sequence |
|---|---|---|
| C3 | 60 | FWD: CACCGCCAAGAATGCCTSC |
| REV: GATCAGGTGTTTCAGCCGC | ||
| CD55 | 58 | FWD: AATGTGGGGAGAGGGAAATC |
| REV: CTGTGGCGATTCTG TTACA | ||
| Cox-2 | 55 | FWD: ATTCAACACACTCTATCACTGGC |
| REV: TGGTCAAATCCTGTGCTCATACAT | ||
| Factor H | 55 | FWD: ACCACATGTGCCAAATGCTA |
| REV: TGTTGAGTCTCGGCACTTTG | ||
| Factor B | 58 | FWD: CCAGCATTTGGGTTTCAGTT |
| REV: CACACCTCCAGAGGAGAAGC | ||
| Ribosomal 18sb | 58 | FWD: CTGGTAATTCATCTCTCTGCTCTG |
| REV: GCGACCAAAGGAACCATAAC |
a
Annealing temperature
b
House-keeping gene to which genes of interest were normalized