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. Author manuscript; available in PMC: 2015 Dec 21.
Published in final edited form as: Sci Signal. 2014 Jul 29;7(336):ra70. doi: 10.1126/scisignal.2005275

Fig. 6. Roles of the transmembrane domain and cytosolic tail of TMED7 in TLR4 signaling.

Fig. 6

(A to D) HEK 293T cells were transfected with reporter plasmids for (A and C) NF-κB or (B and D) IFN-β together with the indicated amounts (ng) of plasmid encoding (A and B) the HA-tagged Delta mutant or (C and D) the HA-tagged CC mutant. 48 hours after transfection, cells were left untreated or were treated with LPS (10 ng/ml) for 6 hours and then were subjected to luciferase assays as described in the Materials and Methods. Data are the mean firefly luciferase activity normalized to Renilla luciferase activity from a single experiment, which is representative of five independent experiments. Error bars represent the SD of three technical replicates.