Table 3. Genetic Incorporation of Azobenzene–Alanine Analogues into Superfolder GFP.
| compd | X | R | calcd mass, Da | obsd mass, Da | yield (mg/L) | % Gln incorpa |
|---|---|---|---|---|---|---|
| 4d | H | 3-CN | 27856.3 | 27857.6 ± 1.3 | 32.0 | 0 |
| 4g | H | 2,6-F2 | 27868.1 | 27867.6 ± 1.0 | 22.3 | 0 |
| 4h | H | 2,4,6-F3 | 27886.1 | 27885.2 ± 0.9 | 31.6 | 0 |
| 4k | H | 2,3,4,5,6-F5 | 27921.3 | 27920.3 ± 1.8 | 5.4 | 36 |
| 4l | F | 2,6-F2 | 27886.1 | 27886.0 ± 1.1 | 10.2 | 4 |
| 4m | F | 2,4,6-F3 | 27903.3 | 27901.9 ± 1.6 | 3.9 | 21 |
| 4n | F | 2,3,4,5,6-F5 | 27941.1 | 27940.3 ± 1.7 | 4.8 | 50 |
| 4o | Cl | 2,6-F2 | 27902.1 | 27904.6 ± 1.6 | 7.8 | 68 |
| 4p | Cl | 2,4,6-F3 | 27920.5 | 27920.3 ± 1.2 | 4.4 | 27 |
a
The Gln incorporation into sfGFP in the purified protein samples was calculated on the basis of ion counts using the following equation: Gln % = IsfGFP-S2Q/(IsfGFP-S2Q + IsfGFP-S2→Aba), where IsfGFP-S2Q and IsfGFP-S2→Aba are the ion counts of sfGFP-S2Q and sfGFP-S2 → Aba, respectively, in the deconvoluted mass spectra.