Fig 1. The retromer subcomplex MoVps35-MoVps26-MoVps29 is required for proper pathogenesis in the rice-blast fungus.
(A) Pathogenicity assays on rice seedlings. Rice leaves (Oryza sativa cv. CO39) sprayed with conidia from the wild-type strain ΔKu70, ΔMovps35 mutant and the complemented ΔMovps35 strain. (B), (C), (D) are pathogenicity assays on barley leaves. Barley leaves were inoculated with mycelial plugs (B) or with 20 microliter of conidial suspension (ca 105 conidia/mL) (C). (D) Barley infection using wounded/abraded leaves. Lesion formation on leaves was observed 7 days after inoculation with mycelial plugs from the indicated strains. (E) Yeast two-hybrid assay for the interaction between retromer components. (F) and (G) Mutant analysis in MoVPS26 and MoVPS29 suggests a role for cargo-specific retromer in M. oryzae pathogenicity. Conidia from the indicated strains were inoculated on the susceptible rice (CO39) seedlings and imaged 7 days post inoculation.