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. 2015 Dec 10;11(12):e1005704. doi: 10.1371/journal.pgen.1005704

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© 2015 Zheng et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 8 — (A) GFP-trap based pull down experiment indicating the interaction between MoVps35-GFP and RFP-MoAtg8 in the transformant. Total proteins isolated from the wild-type strain (WT) are included as a negative control. MoVps35-GFP shows specific interaction with the cleaved and lipidated MoAtg8 during MM-N induced starvation. Top, middle, and bottom images represent immunoblot detection with anti-RFP, anti-GFP, and anti-actin antibodies, respectively, as indicated. (B) Delayed post-translational processing of MoAtg8 in ΔMovps35 mutant. Immunoblot analysis of total lysates from CM and MM-N cultured (3 h to 9 h in starvation environment) ΔMoatg8 RFP-MoATG8 and ΔMovps35 RFP-MoATG8 transformants, with anti-RFP antibody. Coomassie blue staining of total lysates serves as a loading control.