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. 2015 Nov 19;13(1):769–777. doi: 10.3892/mmr.2015.4588

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Copyright: © Li et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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mRNA expression levels of OPG and RANKL in bone tissue were measured by reverse transcription-quantitative polymerase chain reaction. Data were analyzed using the 2^−ΔΔCq method and normalized to β-actin. (A) The OPG mRNA relative ratio in vivo. (B) The RANKL mRNA relative ratio in vivo. (C) The OPG mRNA relative ratio in vitro. (D) The RANKL mRNA relative ratio in vitro. Values are presented as the mean ± standard deviation. ^*P<0.05, ^**P<0.01. OPG, osteoprotegerin; RANKL, receptor activator of nuclear factor-κB ligand; OVX, ovariectomized rats; GaN, gallium nitrate; NS, not significant.