Table 1. Primer and TAQMAN probe sequences used for real-time PCR.
PDK4, pyruvate dehydrogenase kinase 4; PGC-1α, peroxisome proliferator-activated receptor-γ coactivator-1α CS, citrate synthase; Cyt c, cytochrome c oxidase, HKII, hexokinase II; GLUT4, glucose transporter-4; PGC-1β, peroxisome proliferator-activated receptor gamma coactivator-1β FOXO1, forkhead box O1.
| Gene | Primer sequence (forward and reverse) | TaqMan Probe |
|---|---|---|
| PDK4 | 5'-TCCACTGCACCAACGCCT-3' | 5'-ATAATTCCCGGAATGCTCCTTTGGCTG-3' |
| 5'-TGGCAAGCCGTAACCAAAA-3' | ||
| PGC-1α | 5’-CAAGCCAAACCAACAACTTTATCTCT-3’ | 5’-AGTCACCAAATGACCCCAAGGGTTCC-3’ |
| 5’-CACACTTAAGGTGCGTTCAATAGTC-3’ | ||
| CS | 5’-GACTACATCTGGA ACACACTCAACTCA-3' | 5'-ACGGGTTGTTCCAGGCTATGGCCA-3' |
| 5'-CGCGGATCAGTCTTCCTTAGTAC-3' | ||
| Cyt c | 5'-GGTCTCTTTGGGCGGAAGAC-3’ | 5'-CCCTGGATACTCTTACACAGCCGCCAA-3' |
| 5' CTCTCCCCAGATGATGCCTTT 3’ | ||
| HKII | 5'-TTGTCCGTAACATTCTCATCGATT-3' | 5'-ACCAAGCGTGGACTGCTCTTCCGA-3' |
| 5'-TGTCTTGAGCCGCTCTGAGAT-3' | ||
| GLUT4 | 5'-CCTGCCAGAAAGAGTCTGAAGC-3' | 5'-CAGAAACATCGGCCCAGCCTGTCA-3' |
| 5'-ATCCTTCAGCTCAGCCAGCA-3’ | ||
| PGC-1β | 5'-GAGGGCTCCGGCACTTCT-3' | 5'-CCCAGATACACTGACTACGATTCCAATTCAGAAG-3' |
| 5'-CATGGCTTCATACTTGCTTTTCC-3' | ||
| FOXO1 | 5'-ACCGAACAGGATGATCTTGGA-3' | 5'-CCATCTGCCGCAAAGATGGCCTCTA-3' |
| 5'-TTGCTTATCTCAGACAGACTGGGTAA-3' |