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. 2015 Nov 27;13(1):605–612. doi: 10.3892/mmr.2015.4619

Figure 2.

Figure 2

Overexpression of Tat-interacting protein (TIP)30 inhibits glioma cell growth. (A) mRNA expression levels of TIP30 were analyzed by real time-quantitative polymerase chain reaction in lentivirus (LV)-TIP30, mock and LV-control (Con) U87 glioma cells. (B) Protein expression levels of TIP30 were analyzed by western blotting. (C) Overexpression of TIP30 inhibits glioma cell growth. Four days following virus infection, the U87 cells were seeded in 96-well plates in order to carry out an MTT assay. *P<0.05 LV-TIP30 group, vs. the LV-Con group. (D) Cell apoptosis was detected by flow cytometry. The cells were assessed by flow cytometry following Annexin V staining. Q4-2, early apoptotic cells positively stained for Annexin V-fluorescein isothiocyanate (FITC), and negative for propidium iodide (PI); Q3-2, normal cells not stained by Annexin V-FITC or PI; Q2-2, necrotic cells and late apoptotic cells stained by both Annexin V-FITC and PI; (E) Data are presented as the mean ± standard deviation of three independent experiments. *P<0.05 and **P>0.05.