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. 2015 Nov 5;13(1):27–34. doi: 10.3892/mmr.2015.4503

Figure 1.

Figure 1

GAS5 inhibits bladder cancer cell proliferation. (A) Bladder cancer cells were transfected with GAS5-siRNA for 24, 48 or 72 h, and cell viability was examined using the Cell Counting kit-8 assay. (B) Bladder cancer cells were transfected with GAS5-siRNA, and the GAS5 expression levels were detected after 48 h of incubation by reverse transcription-quantitative polymerase chain reaction. (C) Bladder cancer cells were transfected with GAS5-siRNA, and following 48 h of incubation, the relative number of cells in each cell cycle phase was detected by propidium iodide staining and fluorescence-activated cell sorting analysis. Values are expressed as the mean ± standard error of the mean (n=3/group). *P<0.05, compared with the control. OD, optical density; NC, negative control; Mock, scramble siRNA-transfected group; GAS5, growth arrest specific 5; GAS5 siRNA-1 and GAS5 siRNA-2, transfected with GAS5-small interfering RNA.