Figure 3.

Methylene blue prevents neuronal death and promotes autophagy at the perilesional area following TBI. (A and B) Representative images of NeuN-stained coronal tissue sections 24 and 72 h post-TBI. (Scale bar=50 µm). (C) Quantitative analysis revealed a significant increase in the number of NeuN-positive cells 24 and 72 h post-TBI following treatment with MB. The data are presented as the mean ± standard deviaton (n=6; ^*P<0.05). (D and E) Representative images of Beclin 1-stained coronal tissue sections 24 and 72 h post-TBI demonstrating marked cytoplasmic staining in the MB-treated cells (Scale bar=50 µm). MB, methylene blue; TBI, traumatic brain injury; NS, normal saline.