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. 2015 Nov 11;13(1):49–58. doi: 10.3892/mmr.2015.4553

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Copyright: © Hu et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Characterization of nerve growth factor (NGF) and basic fibroblast growth factor (bFGF) lentiviral transfection of bone marrow mesenchymal stem cells (BMSCs). The expression levels of NGF and bFGF proteins in the various transfected cells was determined by western blotting 72 h post-transfection. (A) The plv-NGF-transfected BMSCs and the plv-NGF-bFGF co-transfected BMSCs expressed NGF protein, whereas the remaining groups secreted little NGF protein. The plv-bFGF-transfected BMSCs and the plv-NGF-bFGF co-transfected BMSCs expressed bFGF protein, whereas the remaining groups secreted little bFGF protein. (B) The data are presented as the mean ± standard deviation (SD) from five independent groups (n=3). ^**P<0.01, as compared with the untransfected BMSCs, the plv-blank-transfected BMSCs, and the plv-bFGF-transfected BMSCs. (C) The data are presented as the mean ± SD (n=3). ^**P<0.01, as compared with the untransfected BMSCs, the plv-blank-transfected BMSCs, and the plv-NGF-transfected BMSCs.