Table II.
Labeling efficiency quality control.
| Sample ID | Dye name | Dye (pmol/µl) | cRNA concentration (µg/µl) |
Specific activitya (pmol dye/µg cRNA) |
Volume (µl) | Total quantity (µg) |
|---|---|---|---|---|---|---|
| 2 | Cy3 | 16.69 | 0.74462 | 22.41412 | 20 | 14.892 |
| 3 | Cy3 | 16.79 | 0.72459 | 23.17172 | 20 | 14.492 |
| 4 | Cy3 | 16.71 | 0.76486 | 21.84714 | 20 | 15.297 |
| 10 | Cy3 | 16.59 | 0.72675 | 22.82766 | 20 | 14.535 |
| 13 | Cy3 | 16.13 | 0.71639 | 22.51567 | 20 | 14.328 |
| 16 | Cy3 | 16.85 | 0.76996 | 21.88425 | 20 | 15.399 |
| 20 | Cy3 | 16.77 | 0.74127 | 22.62334 | 20 | 14.825 |
| 21 | Cy3 | 16.36 | 0.79604 | 20.55173 | 20 | 15.921 |
| 26 | Cy3 | 16.31 | 0.70736 | 23.05757 | 20 | 14.147 |
a
For two-color samples, if yield <825 ng and specific activity <8.0 pmol Cy3 or Cy5/µg cRNA, hybridization did not proceed and the cRNA was re-prepared. For one-color samples, if yield <1.65 µg and specific activity <9.0 pmol Cy3 or Cy5/µg cRNA, hybridization did not proceed and the cRNA was re-prepared.