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. Author manuscript; available in PMC: 2015 Dec 21.
Published in final edited form as: J Genet Genomics. 2015 May 14;42(6):319–330. doi: 10.1016/j.jgg.2015.03.012

Fig. 1. Illustration of sof mutants screening.

Fig. 1

A: Schematic diagram of the T-DNA constructs in the binary vector pTA7002, which confers hygromycin resistance in plants. B: RNA blot analysis with FREE1 probe detected accumulated FREE1-siRNA with DEX application in FREE1-RNAi line. Detection of U6 served as loading control. C: Immunoblot analysis with anti-FREE1 antibody. FREE1 protein level was barely detected in FREE1-RNAi plants with DEX treatment. Antibodies against the protein cFBPase served as the loading control. D: Diagram showing the seedling lethal phenotype caused by FREE1 loss of function. E: Schematic illustration of screening procedure. Single insertion Dex-inducible FREE1-RNAi seeds were mutated with EMS, and with DEX induction survived M2 seedlings were selected as putative sof mutants. F: Example of primary screening with M2 seeds plated on MS plates supplied with 10 μmol/L DEX. Scale bar, 5 mm.