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. Author manuscript; available in PMC: 2015 Dec 21.
Published in final edited form as: J Genet Genomics. 2015 May 14;42(6):319–330. doi: 10.1016/j.jgg.2015.03.012

Fig. 3. Vacuole morphology analysis of representative FREE1-related sof mutants.

Fig. 3

A: Vacuole morphology was analyzed using FM 4-64 dye staining. Compared to the wild type plants, fragmented vacuoles were observed in FREE1-RNAi plants. FREE1-related sof mutants sof220 and sof641 showed large central vacuoles. B: The short-time DAPI staining was used to visualize the cell shape. C: Merged result of FM 4-64 dye staining and DAPI staining. Scale bar, 25 μm. D: 3 × enlargement of merged images. Arrows indicated the fragmented vacuoles in FREE1-RNAi plants.