. Author manuscript; available in PMC: 2015 Dec 21.

Published in final edited form as: J Stem Cell Regen Biol. 2015 Nov 5;1(1):http://www.ommegaonline.org/article-details/Gene-Editing-in-Human-Pluripotent-Stem-Cells---Choosing-the-Correct-Path/630.

Table 1.

A comparison of site-specific nucleases that can be used for gene editing, range from low (+) to high (+++++).

	ZFNs	TALENs	CRISPR/Cas9	RFNs
Cost	+++++	+++	++	++
Reagent design limitations	+++	+	++	+++
Reagent development difficulty	+++	+++	+	++
Efficiency	+++	++++	++++	+++
Off-target effects	++	++	+++++	+

Abbreviations: ZFNs, Zinc-Finger Nucleases; TALENs, Transcription Activator-Like Effector Nucleases; CRISPR, Clustered Regularly Interspaced Short Palindromic Repeats; RFNs, dimeric CRISPR RNA-guided FokI nucleases.