Figure 1. Schematic of profound shock model.
Animals after cannulation underwent hemorrhage form 5 minutes reduce blood pressure to a mean arterial pressure of less than 35 mmHg, 30 minutes of shock, 5 minutes of resuscitation, and 15 minutes of observation. During resuscitation animals were randomized to either plasma or saline with an initial 10% estimated blood volume bolus followed by an addition as needed saline resuscitation to obtain a mean arterial pressure greater than 30.
MAP= mean arterial pressure in mmHG