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. 2004 Apr 7;6(4):R284–R290. doi: 10.1186/bcr785

Figure 4.

Figure 4

Detection of BRCA2 999del5 protein under denaturing conditions and after proteasome inhibition. (a) COS7 cells were transfected with pIND-BRCA2 999del5 or pIND-LacZ without proteasome inhibition. Samples were treated under denaturing conditions. (b) COS7 cells were transfected with pIND-BRCA2 999del5 or pIND-LacZ with proteasome inhibition. Samples were purified with nickel-coated agarose beads under denaturing conditions. Samples in both (a) and (b) were blotted with an antibody against the V5 tag (α-V5).