Fig 2. Intravital imaging of transmigrated B. burgdorferi in the knee joint-proximal tissue of a living mouse.
Infectious GFP-expressing (green) B. burgdorferi (left and right panels GCB726, central panel GCB966, 4x108 spirochetes per mouse) were injected into the tail vein of Cd1d -/- mice. At 20 hours post-inoculation, vascular transmigration was scored in the knee joint-proximal tissue of living mice by intravital microscopy using a spinning disk laser confocal microscope. Blood vessels were stained with Alexa Fluor 555-conjugated PECAM-1 antibody (red). Video footage of transmigrated spirochetes is shown in S1 and S2 Videos.