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. 2015 Dec 21;10(12):e0142699. doi: 10.1371/journal.pone.0142699

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© 2015 Wiberg et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Fig 3 — (A) The medial gastrocnemius muscles and the soleus muscles were harvested and fast frozen in liquid nitrogen for subsequent qRT-PCR analysis of myoD expression 7 and 28 days after nerve transection (cut) or crush injury. **P<0.01, ***P< 0.001 represents statistically significant difference to the respective control (unoperated group) muscles. Two-Way ANOVA indicates that the soleus and gastrocnemius muscles are significantly (P<0.001) different for each type of injury. (B) Control and soleus muscles harvested from animals 7 days after nerve crush injury were stained with antibodies directed against dystrophin (outline of muscle fibre) and myoD (green). Arrows highlight 5 representative nuclei (DAPI staining) which correspond with myoD positive staining.